Flowers on the Road (1961)

Yae and Kiku, daughters of drapers in Edo, must travel to Kyoto in place of their sickly fathers to attend to some businesses affairs. On their way to Kyoto, the two ladies are ambushed by a group of bandits. However a good Samaritan named Santaro comes to their rescue. Santaro is aiding a young samurai named Sanshiro who is on an official mission to deliver a message to a noble in Kyoto. When Yae and Kiku discover that a gang of assassins is after Sanshiro, they decide to help Sanshiro to complete his mission.